Am J Clin Pathol. 2005 Jun;123(6):826-32.
Diagnostic usefulness of aberrant CD22 expression in differentiating neoplastic
cells of B-Cell chronic lymphoproliferative disorders from admixed benign B
cells in four-color multiparameter flow cytometry.
Huang J,
Fan G,
Zhong Y,
Gatter K,
Braziel R,
Gross G,
Bakke A.
Department of Pathology and Laboratory Medicine,
The diagnosis of B-cell chronic lymphoproliferative disorders is a great
challenge when made in a background of polyclonal B cells. We studied the
diagnostic usefulness of aberrant CD22 expression for differentiating neoplastic
from benign B cells by 4-color flow cytometry. Of 56 cases of B-cell chronic
lymphoproliferative disorders, we found that neoplastic cells showed aberrant
CD22 expression in 39 (70%) of 56 cases, including chronic lymphocytic leukemia,
mantle cell lymphoma, marginal zone lymphoma, hairy cell leukemia, and
follicular lymphoma. In 4 cases, monoclonality was detected definitively only by
evaluating the immunoglobulin light chain restriction in B cells with aberrant
CD22 expression because numerous polyclonal B cells were present. Aberrant CD22
expression is a useful marker for detection of monoclonal B cells admixed with
numerous benign polyclonal B cells.
PMID: 15899772 [PubMed - indexed for MEDLINE]